David R. Singleton

Administrative Director, Precision Microbiome Engineering Engineering Research Center

Dr. Singleton’s research focuses on beneficial organisms in environmental systems, particularly those associated with the removal or transformation of harmful chemicals in contaminated soils. Particular interests include the identification and study of uncultivated microorganisms relevant to bioremediation processes. Prior research focused on the use of stable-isotope probing (SIP) to identify organisms associated with the removal of aromatic hydrocarbons in soil. He and his students have successfully isolated a number of those organisms and characterized several SIP-identified but previously uncultivated bacteria including the first isolates in the genera Immundisolibacter and Rugosibacter, two ubiquitous soil bacteria lineages heavily involved in the remediation of petroleum-contaminated soils. Current research explores the microbial communities of contaminated sediment systems and evaluating novel methods with the goal of increasing the efficacy of bioremediation in those environments.

Contact Information

  • Office Location: 121 Hudson Hall, Box #90287 Duke University, Durham NC, 27708
  • Office Phone: 1-919-668-9104
  • Email Address: david.singleton168@duke.edu


  • Ph.D., University of Georgia, 2002
  • B.A., Illinois Wesleyan University, 1996